NF1~180mut表达载体构建及其在脑血管细胞中的表达     

Ajigul Mamut  Mamattursun Eziz  Anwar Mohamm  Mattohti Anayit  Atiyagul Rixit 《寒旱区科学》2017,9(5):467-475

目的 前期研究中发现一合并脑血管狭窄的Ⅰ型神经纤维瘤病家系,为探索血管狭窄的病因,本研究构建该家系患者NF1基因共同突变产物NF1~180mut的过表达质粒,观察该质粒在脑血管细胞中的表达。方法 设计NF1~180mut全长序列的相应引物,采用pEGFP-N1作为载体框架,构建pEGFP-N1/NF1~180mut表达载体,通过Western blot法及共聚焦检测其蛋白表达;之后采用脂质体转染重组质粒于脑血管平滑肌细胞或内皮细胞中观察NF1~180mut在两种脑血管细胞中的表达。结果 ①成功构建了NF1~180mut表达载体;②该表达载体可在脑血管内皮细胞及平滑肌细胞内表达;③初步通过细胞计数研究及增殖实验显示NF1~180mut对脑血管平滑肌细胞和血管内皮细胞的生长及增殖有一定的作用。结论 本研究中构建了NF1-Q181X突变后的产物NF1~180mut表达载体,为进一步深入研究NF1~180mut导致脑血管狭窄发生的通路提供了良好的体外研究工具。  相似文献   

Multi-source data fusion and modeling to assess and communicate complex flood dynamics to support decision-making for downstream areas of dams: The 2011 hurricane irene and schoharie creek floods,NY     

《International Journal of Applied Earth Observation and Geoinformation》2017

In light of climate and land use change, stakeholders around the world are interested in assessing historic and likely future flood dynamics and flood extents for decision-making in watersheds with dams as well as limited availability of stream gages and costly technical resources. This research evaluates an assessment and communication approach of combining GIS, hydraulic modeling based on latest remote sensing and topographic imagery by comparing the results to an actual flood event and available stream gages. On August 28th 2011, floods caused by Hurricane Irene swept through a large rural area in New York State, leaving thousands of people homeless, devastating towns and cities. Damage was widespread though the estimated and actual floods inundation and associated return period were still unclear since the flooding was artificially increased by flood water release due to fear of a dam break. This research uses the stream section right below the dam between two stream gages North Blenheim and Breakabeen along Schoharie Creek as a case study site to validate the approach. The data fusion approach uses a GIS, commonly available data sources, the hydraulic model HEC-RAS as well as airborne LiDAR data that were collected two days after the flood event (Aug 30, 2011). The aerial imagery of the airborne survey depicts a low flow event as well as the evidence of the record flood such as debris and other signs of damage to validate the hydrologic simulation results with the available stream gauges. Model results were also compared to the official Federal Emergency Management Agency (FEMA) flood scenarios to determine the actual flood return period of the event. The dynamic of the flood levels was then used to visualize the flood and the actual loss of the Old Blenheim Bridge using Google Sketchup. Integration of multi-source data, cross-validation and visualization provides new ways to utilize pre- and post-event remote sensing imagery and hydrologic models to better understand and communicate the complex spatial-temporal dynamics, return periods and potential/actual consequences to decision-makers and the local population.  相似文献   

顾及尺度变化和数据更新的道路网匹配算法     

郭庆胜  谢育武  刘纪平  王琳  周林 《测绘学报》2017,46(3):381-388

道路网数据匹配是地理空间数据库进行变化探测和数据更新的重要前提,不同比例尺下的道路网之间的匹配是一个非常重要的部分。本文总结和分析了道路网匹配的已有算法,针对不同比例尺道路网之间的匹配可能存在的问题和难点,设计了一个融合多种匹配技术的算法。在考虑不同比例尺下道路网数据的特点基础上,改进了空间场景结构的评价方法;分析了stroke匹配算法在不同比例尺道路网数据下的局限性,提出了一种可针对不同比例尺下道路数据存在变化与更新的stroke部分匹配算法。试验表明,文中所提出的方法能够适应不同比例尺下道路网的匹配,匹配效果较好,运行效率较高。  相似文献   

广州高建筑物雷电回击光脉冲特征分析     

黄晓磊  吕伟涛  武斌  孙秀斌  马颖  陈绿文  齐奇  刘恒毅 《热带气象学报》2021,37(3):439-449

为了深入认识负地闪放电过程中光辐射信号的特性, 对广州高建筑物雷电观测站所获得的回击光脉冲波形进行了分析。对观测到的88例负地闪事件中的184次回击(包括60次下行闪电首次回击、58次下行闪电继后回击、66次上行闪电继后回击)的光脉冲特征进行了统计分析, 结果表明: 下行闪电首次回击光脉冲10%~90%上升时间T₁的算术平均值/中值为32.5/31.4μs, 20%~80%上升时间T₂的算术平均值/中值为22.6/22.4μs, 半峰宽度T₃的算术平均值/中值为131.1/117.0μs。下行闪电继后回击光脉冲T₁的算术平均值/中值为30.4/27.7μs, T₂的算术平均值/中值为19.5/17.6μs, T₃的算术平均值/中值为153.6/142.6μs。在21例下行多回击负地闪事件中, 光脉冲回击间隔时间在12.6~368.6 ms范围之间, 算术平均值为78.7 ms, 有14%闪电事件存在继后回击光脉冲峰值大于首次回击的情况。上行闪电继后回击光脉冲T₁的算术平均值/中值为27.5/24.3μs, T₂的算术平均值/中值为17.0/15.7μs, T₃的算术平均值/中值为132.2/124.5μs。总体上, 下行闪电首次回击的光脉冲上升时间最长、下行闪电继后回击次之、上行闪电继后回击最小; 下行闪电继后回击脉冲半峰宽度比下行闪电首次回击及上行闪电继后回击的更大。   相似文献   

基于结构分析的点状地图符号识别   总被引：1，自引：0，他引：1  

张涛  尹永利  刘肖琳 《测绘工程》2000,9(3):15-18

通过对点状地图符号特点及其识别难度的分析,提出了结构分析为主要识别方法,在结构分析中用模糊编号算法对组成符号的基元进行编码,有效地表示出基元的空间位置关系,大大消除了细化和轻微旋转对提取基元的影响,从而提高了识别率。  相似文献   

EVALUATION OF GROUND STROKE DENSITY DISTRIBUTION AND LIGHTNING DETECTION EFFICIENCY IN BEIJING-TIANJIN-HEBEI AREA   总被引：8，自引：0，他引：8  

Tao Shanchang  Meng Qing 《Acta Meteorologica Sinica》1996,10(3):346-355

It is possible to understand the lightning activities in a specific region and compare test resultsof different apparatus only when a reliable evaluation of detection efficiency distribution pertainingto a particular lightning location system(LLS)is available.Based on the data in 1992.an approximate evaluation of detection efficiency spatial distribu-tion for single-station lightning location system(M-LDARS)and LLP three-station lightning loca-tion system in Beijing-Tianjin-Hebei area is presented in the paper,showing that the average detec-tion efficiencies are smaller than 48% and 46% respectively.In addition,the article offers an eval-uation of spatial distribution of ground stroke density(D_g)and positive stroke percentage,indicat-ing that six high stroke density zones exist along the southeastern sides of the Taihang and Yan-shan Mountains.The stroke density of mountainous region is higher than that of the plain region,in contrast to the case of positive stroke percentage.Also,it is shown that within a radius of 250km,the average of D_g is 1.2(km~(-2) a~(-1))while the average positive stroke percentage is 10.9%.Finally,the paper proposes a possible test method of accurately evaluating the spatial distributionof detection efficiency(A).  相似文献   

北京与兰州地区的地闪特征   总被引：10，自引：8，他引：10  

郄秀书  郭昌明 《高原气象》1990,9(4):388-394

利用闪电定位系统(LLS)在兰州地区和北京地区探测到的共两万多个地闪资料,对两地的正、负地闪特征进行了对比。分析发现兰州地区的正闪比例是北京地区正闪比例的5倍多。北京地区的正闪平均强度近似为负闪的3倍,而兰州地区的正闪强度只有负闪的2.2倍。无论正闪还是负闪,两地的地闪强度均是稳定的正态分布,并且与雷暴本身的特征无关,从而可用DF强度来作雷暴临近的估计。两地的正、负地闪的回击数均按指数规律下降,不过北京地区的多次回击较兰州地区的多次回击要多。两地的闪电频数都在下午后期出现峰值,但北京地区的闪电频数最大值和最小值都较兰州地区早1小时出现。 通过试验找到了地闪定向仪显示数与闪电辐射电场和放电电流的关系,扩大了地闪定向仪的利用率。  相似文献   

CZ-1200型冲击钻机的开发与应用          下载免费PDF全文

高甲  陈毅斌  王振奎 《探矿工程》2002,(2):33-34

针对目前国内防渗墙、灌注桩桩孔施工及水井钻凿的主要设备CZ－22型钢绳冲击式钻机在使用过程中的一些不足之处，研制开发了CZ－1200型冲击钻机。介绍了钻机的结构特点及其在防渗墙、水井施工中的应用。  相似文献   

晚冰期Younger Drayas环境灾变   总被引：3，自引：0，他引：3  

黄春长 《地球科学进展》1998,13(4):356-363

新仙女木事件(Younger Dryas Event)是过去15000年里全球最为严重的环境灾变事件。它使得冰期气候急剧回返,陆地自然环境严重恶化,并且遣成更新世末期的生物界大绝灭。该事件在深海沉积、大陆冰盖和湖泊沉积物剖面都有多种十分显著的记录。对其成因和机制的研究,将会深入揭示太阳辐射—大气—海洋气—冰盖气—陆地气—生物圈相互作用系统中的非线性反馈现象,为探索人类面临的全球变化问题提供借鉴。  相似文献   

Tsunamigenic incisions produced by the December 2004 earthquake along the coasts of Thailand, Indonesia and Sri Lanka     

Sergio Fagherazzi  Xizhen Du 《Geomorphology》2008,99(1-4):120-129

Field observations and satellite images indicate that tsunami waves exhibit specific patterns during flooding and recession forming characteristic incisions in the coastal landscape. To study these incisions we analyze high resolution remote sensing images of the coastline of Indonesia, Thailand and Sri Lanka impacted by the tsunami of December 26th, 2004. The analysis sheds light on the different mechanisms by which currents scour incisions during the flooding and receding phases of a tsunami. During flooding the high velocity flow indents the levees of existing tidal channels and bays, leaving short flood scours. The receding water then dissects the coastline with equally spaced return channels widening toward the coast.  相似文献